Imaging-based Systems Biology Laboratory

The central dogma of biology states that the genome is the blueprint of life and the source from which information flows to define the final phenotype. However, in multicellular organisms, all cells contain identical copies of the genome yet exhibit drastically variable phenotypes. Vital to understanding complex diseases is the realization that in living systems, new properties emerge that cannot be explained by the sum of their building blocks and can causally impact their behavior. For example, studying cells in isolation cannot give us an idea about an individual’s immune response or why some cancers resist therapy.

We are interested in understanding and deriving basic “systems properties” such as cell relationships, interconnectivity, causality, and response to perturbation. With this regard, we believe that breakthroughs in biomedical research can only happen if scientists are empowered with tools that allow them to “see” life. Microscopy-based techniques and quantitative methods based on advanced computer vision and data-driven modeling will be the primary approaches to systematically map the spatial localization, temporal dynamics, and activity profiles of proteins and nucleic acids within and across thousands of single cells.

Our focus lies primarily on pediatric diseases and the interplay between the body’s immune system and its ability to fight cancer (Immuno-oncology). We are also interested in immune disorders that can clinically manifest as increased susceptibility to infections, malignant diseases, or immune dysregulation.