Introduction to Proteomics & Protein Biology

This course introduces participants to the basic biology of proteins and proteomics. Proteomics is an advanced science that looks into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. The course covers the two major aspects of proteomics i.e., Gel-based Proteomics and Mass Spectrometry-based Proteomics. The Gel-based Proteomics covers different techniques; i.e. SDS-PAGE, 2-DE, 2D-DIGE etc., that had a major contribution in transition from protein chemistry to proteomics.

Mass Spectrometry, on the other hand, is an advanced analytical technique for accurate mass measurement. In this latest module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry.

The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics. Importantly, the course

The goal of this course is to develop participants knowledge in the field of proteomics as well as, introduce them to the current trends within the protein technology and proteomics field. Additionally, the course aims at providing participants with the experience needed to interpret and present research.

Upon completion of this course, participants will be able to:

• Understand the importance of proteomics as a part of the evolutionary omics technology
• Design, analyze, evaluate a proteomic experiment
• Be familiar with liquid chromatography, mass spectrometer
• Understand the difference between genome and proteome in terms of biology and complexity
• Select the appropriate proteomics platform based on a given hypothetical biological question to be elucidated.
• Acquire practical and theoretical knowledge in proteomics
• Understanding how to identify proteins from mass spectrometry data
• Understand the analysis of post-translational modifications and protein-protein interactions
• Acquire on-hands experience with in-solution digestions, LC-ESI mass spectrometry and protein identification.

Prof. Sameh Magd Eldin; PhD. Head of proteomics research program unit; Basic research department.

Prof. Sameh Magd Eldin; DVM., PhD. Head of proteomics research program unit; Basic research department.

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