The proteomics and metabolomics research program at Hospital 57357, the first of its kind in Egypt and the Middle East, is equipped with top-notch facilities and empowered with a fine selection of specialized scientists with solid experience.
The program aims at empowering research and clinical diagnostic capabilities by precise qualitative and quantitative protein identification using high-end state- of- art proteomics technology. The program offers several specialized and comprehensive services, solutions and packages. Their uniqueness stems from the fact that they are simply the only ones offering these services in Egypt. Costs include reagents, sample processing, manpower, instrument usage, data analysis and reporting. .
The proteomics and metabolomics program believes that innovation is a group endeavor. That is why it is collaborating with the top scientists in Egypt and worldwide. Their collaboration is expanding and they are always welcoming a powerful collaboration targeting high impacted scientific journals. Present collaborations include: Zewail city of science and technology, Armed forces college of Medicine, Urology and nephrology center, Mansoura, Baylor College of Medicine (USA), University of Rochester (USA), Niigata University (Japan), and Fred Hutchinson cancer research center (USA).
Services and Costs
We offer a unique service with different assay length and applications. Our uniqueness stems from the fact that we are simply the only ones offering these services in Egypt. Costs include reagents, sample processing, manpower, instrument usage, data analysis and reporting.
General Workflow
We work professionally according to world standards to ensure accurate, sensitive results. For all samples, we perform an efficient protein extraction using varieties of extraction buffers, quantification of proteins and appropriate protein reduction and alkylation prior to trypsinization. All samples are subjected to C18 desalting step to make sure purified peptides are going to our clean TripleTOF 5600+.
Data analysis
- Data is searched by up to two tandem mass spectrometry protein identification algorithms, including X!Tandem, OMSSA, comet, tide, andromeda.
- Label free quantification based on spectral abundance “NSAF or emPAI” or area under the curve “AUC” is provided free of charge.
- Samples or groups of samples are compared, generating fold-change values for every measured protein.